Non-invasive assessment of adrenocortical activity as a measure of stress in giraffe (Giraffa camelopardalis)

BMC Veterinary Research

Table 3 Assay comparison for the ACTH challenge tests

		Enzyme immunoassay targeting
Animal	Measure	Cortisol	Cortico-sterone	11,17- DOA	3α,11-oxo-CM	3β,11β-diol-CM	3α,11β-diol-CM
Giraffe 1^a	Latency to Peak	13.5 h	50.5 h	13.5 h	11.5 h	13.5 h	30.0 h
	Peak Duration (no. of peak samples)	6 h n = 4	<2 h n = 1	37 h n = 24	39 h n = 25	22 h n = 12	5 h n = 3
	Fold Increase (X_peak/M_baseline)	2.6	1.6	33.4	18.2	4.1	2.6
	Z _peak	7.4 sd	2.2 sd	64.4 sd	79.6 sd	13.6 sd	3.4 sd
Giraffe 2^b	Latency to Peak	no peak	no peak	20.5 h	24.0 h	23.2 h	no peak
	Peak Duration (no. of peak samples)			7 h n = 4	<5 h n = 1	5 h n = 3
	Fold Increase (X_peak/M_baseline)			9.6	2.9	2.9
	Z _peak			17.1 sd	3.3 sd	11.3 sd

^aFor Giraffe 1, fecal samples collected from every defecation from 12 h pre-injection to 72 h post-injection
^bFor Giraffe 2, fecal samples collected daily (between 8 am and 10 am) for 5 days before and 4 days after injection, as well as from every defecation between 20 h and 28 h post-injection

ISSN: 1746-6148