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Table 3 Assay comparison for the ACTH challenge tests

From: Non-invasive assessment of adrenocortical activity as a measure of stress in giraffe (Giraffa camelopardalis)

   Enzyme immunoassay targeting
Animal Measure Cortisol Cortico-sterone 11,17- DOA 3α,11-oxo-CM 3β,11β-diol-CM 3α,11β-diol-CM
Giraffe 1a Latency to Peak 13.5 h 50.5 h 13.5 h 11.5 h 13.5 h 30.0 h
Peak Duration (no. of peak samples) 6 h
n = 4
<2 h
n = 1
37 h
n = 24
39 h
n = 25
22 h
n = 12
5 h
n = 3
Fold Increase (Xpeak/Mbaseline) 2.6 1.6 33.4 18.2 4.1 2.6
Z peak 7.4 sd 2.2 sd 64.4 sd 79.6 sd 13.6 sd 3.4 sd
Giraffe 2b Latency to Peak no peak no peak 20.5 h 24.0 h 23.2 h no peak
Peak Duration (no. of peak samples)    7 h
n = 4
<5 h
n = 1
5 h
n = 3
Fold Increase (Xpeak/Mbaseline)    9.6 2.9 2.9
Z peak    17.1 sd 3.3 sd 11.3 sd
  1. aFor Giraffe 1, fecal samples collected from every defecation from 12 h pre-injection to 72 h post-injection
  2. bFor Giraffe 2, fecal samples collected daily (between 8 am and 10 am) for 5 days before and 4 days after injection, as well as from every defecation between 20 h and 28 h post-injection