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Table 2 E. coli isolates and pathological findings in reproductive tract

From: High genetic diversity among extraintestinal Escherichia coli isolates in pullets and layers revealed by a longitudinal study

Farm/flock

Isolate identificationa

Age (weeks)

Reproductive lesions

Serotype

PFGE type

Rearing farm 1

 RI

RI-1

18

no

ntb

LA23

RI-2

no

nt

nt

RI-3

no

nt

LA20

RI-4

no

nt

LA19

 L1/I-A

L1/I-A-1

42

no

nt

S31

L1/I-A-2

no

nt

S37

L1/I-A-5

no

O78:K80

S6

 L2/I-A

L2/I-A-1

42

no

O1:K1

S2

L2/I-A-5

no

O1:K1

S15

 L2/I-B

L2/I-B-4

77

oophoritis

nt

S22

L2/I-B-5

oophoritis

nt

S23

 L3/I-B

L3/I-B-1

77

oophoritis and salpingitis

O2:K1

S19

L3/I-B-2

oophoritis

O2:K1

S19

Rearing farm 2

 RII

RII-1

19

no

O1:K1,O2:K1,O78:K80

Sa3

RII-2

no

O1:K1,O2:K1,

Sa1

RII-3

no

nt

Sa4

RII-4

no

O1:K1

Sa5

RII-5

no

nt

Sa2

 L1/II-A

L1/II-A-1

40

no

O1:K1

nt

L1/II-A-2

no

O1:K1

nt

L1/II-A-3

no

O1:K1

nt

L1/II-A-4

no

O1:K1

nt

L1/II-A-5

no

O1:K1

nt

 L2/II-A

L2/II-A-1

38

degeneration of oviduct

O1:K1,O2:K1,

nt

L2/II-A-2

no

O1:K1,O2:K1,

LA10

L2/II-A-3

oophoritis

O2:K1

LA6

L2/II-A-4

oophoritis

O1:K1,O2:K1,O78:K80

LA9

L2/II-A-5

oophoritis

O1:K1,O2:K1,

LA2

 L1/II-B

L1/II-B-1

80

egg peritonitis

nt

Ca4

L1/II-B-2

no

O2:K1

Ca2

L1/II-B-3

egg peritonitis

nt

Ca3

L1/II-B-4

egg peritonitis

nt

Ca5

L1/II-B-5

egg peritonitis

nt

Ca1

 L2/II-B

L2/II-B-1

68

oophoritis

nt

LA17

L2/II-B-2

oophoritis

nt

LA18

L2/II-B-3

oophoritis

nt

LA18

L2/II-B-4

oophoritis

nt

LA7

L2/II-B-5

oophoritis

nt

LA18

Rearing farm 3

 RIII

RIII-1

18

no

nt

S16

RIII-2

no

nt

S5

RIII-3

no

nt

S5

RIII-4

no

O1:K1,O2:K1,

S4

 L1/III-A

L1/III-A-1

39

oophoritis

nt

B15

L1/III-A-3

no

O1:K1

B14

L1/III-A-5

oophoritis

nt

B3

 L2/III-A

L2/III-A-1

39

no

nt

B4

L2/III-A-2

no

O1:K1

B6

L2/III-A-3

no

nt

B4

L2/III-A-4

no

nt

B8

L2/III-A-5

no

nt

B8

 L3/III-A

L3/III-A-1

39

oophoritis

nt

B1

L3/III-A-2

oophoritis

nt

B1

L3/III-A-3

oophoritis

nt

B1

L3/III-A-4

oophoritis

nt

B1

L3/III-A-5

oophoritis

nt

B17

 L1/III-B

L1/III-B-2-ovary1

67

egg peritonitis

O1:K1

B13

L1/III-B-2-ovary2

egg peritonitis

nt

B12

L1/III-B-3-ovary

no

O1:K1

B12

L1/III-B-3-oviduct

no

nt

B12

L1/III-B-4-oviduct1

no

nt

B5

L1/III-B-4-oviduct2

no

nt

B11

L1/III-B-5

degeneration of ovary and oviduct

nt

B10

 L2/III-B

L2/III-B-1

67

no

O1:K1

B7

L2/III-B-2

egg peritonitis

O1:K1

B7

L2/III-B-3

degeneration of ovary and oviduct

O1:K1

B9

L2/III-B-4

egg peritonitis

O1:K1

B9

 L3/III-B

L3/III-B-3

67

no

O1:K1

B16

L3/III-B-4

oophoritis

O1:K1

B2

L3/III-B-5

no

O1:K1

nt

Rearing farm 4

 RIV

RIV-1

17

no

nt

Ua2

RIV-2

no

nt

Ua1

RIV-3

no

nt

Ua1

RIV-4

no

nt

Ua3

RIV-5

no

nt

Ua4

 L1/IV-A

L1/IV-A-1

41

no

nt

LA16

L1/IV-A-2

no

nt

LA15

L1/IV-A-3

no

nt

LA15

L1/IV-A-4

no

nt

LA27

 L2/IV-A

L2/IV-A-2

41

no

O1:K1

LA13

 L3/IV-A

L3/IV-A-1

41

degeneration of ovary and oviduct

nt

LA21

L3/IV-A-2

egg peritonitis

nt

LA21

L3/IV-A-3

degeneration of ovary and oviduct

nt

LA11

L3/IV-A-4

degeneration of ovary and oviduct

nt

LA11

L3/IV-A-5

no

nt

LA4

 L1/IV-B

L1/IV-B-1

73

degeneration of ovary and oviduct

nt

LA25

L1/IV-B-2

oophoritis

nt

LA1

L1/IV-B-3

oophoritis

nt

nt

L1/IV-B-4

oophoritis

nt

LA5

L1/IV-B-5

oophoritis

nt

LA25

 L2/IV-B

L2/IV-B-1-ovary

73

oophoritis

nt

LA25

L2/IV-B-1-liver

oophoritis

O1:K1

LA25

L2/IV-B-2

oophoritis

O1:K1

LA25

L2/IV-B-3

oophoritis

O1:K1

LA26

L2/IV-B-4

oophoritis

O1:K1

LA25

L2/IV-B-5

oophoritis

nt

LA28

 L3/IV-B

L3/IV-B-1

73

oophoritis

O1:K1

LA3

L3/IV-B-2

oophoritis

O1:K1

LA14

L3/IV-B-3

oophoritis

O1:K1

LA25

L3/IV-B-4

oophoritis

nt

LA8

L3/IV-B-5

oophoritis

O1:K1

LA25

 L2/IV-Z1

L2/IV-Z1-1

31

oophoritis

O1:K1,O2:K1,O78:K80

LA22

 

L2/IV-Z1-2

 

no

O1:K1,O2:K1,O78:K80

LA22

 

L2/IV-Z1-3

 

egg peritonitis

O1:K1,O2:K1,O78:K80

LA22

 L3/IV-Z1

L3/IV-Z1-2

30

degeneration of ovary and oviduct

O1:K1,O2:K1,

LA24

 L3/IV-Z2

L3/IV-Z2-1

33

no

nt

LA12

Rearing farm 5

 RV

RV-3

16

no

O1:K1,O2:K1,

nt

RV-4

no

O1:K1

S7

RV-5

no

O1:K1

S7

 L1/V-A

L1/V-A-2

37

no

O1:K1

nt

L1/V-A-3-oviduct

no

nt

S1

L1/V-A-4-oviduct

egg peritonitis

nt

S18

L1/V-A-4-ovary

egg peritonitis

O1:K1

S7

L1/V-A-6

egg peritonitis

O1:K1

nt

 L1/V-B

L1/V-B-1

64

egg peritonitis

O2:K1

S8

L1/V-B-2

egg peritonitis

O2:K1

S9

L1/V-B-3

egg peritonitis

nt

S25

L1/V-B-5

egg peritonitis

O2:K1

S9

 L2/V-A

L2/V-A-3

39

degeneration of ovary

nt

S27

L2/V-A-4

degeneration of ovary

O78:K80

S29

L2/V-A-5

no

nt

S3

 L2/V-B

L2/V-B-1

74

no

O1:K1

S10

L2/V-B-2

no

O1:K1

S10

L2/V-B-3

oophoritis and salpingitis

O1:K1

S10

L2/V-B-4

oophoritis

nt

S20

L2/V-B-5-ovary

egg peritonitis

O1:K1

S34

L2/V-B-5-heart

egg peritonitis

O1:K1

S34

Rearing farm 6

 L1/VI-A

L1/VI-A-1

38

no

nt

S36

L1/VI-A-2

no

nt

S21

L1/VI-A-4

oophoritis

nt

S11

L1/VI-A-5

no

nt

S21

 L1/VI-B

L1/VI-B-1-ovary

80

degeneration of ovary and oviduct

O1:K1

S33

L1/VI-B-1-oviduct

degeneration of ovary and oviduct

nt

S12

L1/VI-B-2

degeneration of ovary and oviduct

O1:K1

S33

L1/VI-B-3

oophoritis

O1:K1

S26

L1/VI-B-4

degeneration of ovary and oviduct

nt

S24

L1/VI-B-5

egg peritonitis

O1:K1

S35

 L2/VI-A

L2/VI-A-1

38

no

nt

S17

L2/VI-A-2

no

nt

S13

L2/VI-A-4

oophoritis

nt

S32

L2/VI-A-5

oophoritis, degeneration of oviduct

nt

S17

 L2/VI-B

L2/VI-B-2-ovary

80

oophoritis

nt

S28

L2/VI-B-2-oviduct

oophoritis

O1:K1

S30

L2/VI-B-4

degeneration of ovary

nt

S14

L2/VI-B-5

egg peritonitis

O1:K1

S32

  1. Age of birds, lesions in the reproductive tract, serotypes and PFGE types of each E. coli isolates are provided in the corresponding vertical line
  2. aisolates identification: isolates from rearing farms are marked with letter “R” along with farm number and bird number. Likewise, isolates from layer flocks are labelled with letter “L” along with flock number/corresponding rearing farm number – time of sampling (A: peak of production, B: end of production, Z1 or Z2: first or second additional samplings) – bird number – organs (only in those birds from where two samples were collected)
  3. bnon-typeable