Table 1 CD4 exonic primer sets for DNA sequencing
From: Identification and characterization of two CD4 alleles in Microminipigs
Amplification exon | Forward primer sequence | Reverse primer sequence | Length of PCR product size (bp) |
|---|---|---|---|
2 | 5'-GTACCTGTGGGTGTCAGTTTAGAG-3' | 5' -CTTACCCAGCACCAGATATTTTTC-3' | 383 |
3 | 5'-CTCAGACTCAAACTGGGATGATTG -3' | 5' -GATCCCAGAGTTTACTAGGAGCTG-3' | 366 |
4 | 5'-AATGAGCAACTCAGATCAGAAGAGT-3' | 5'-CTTATCCATCTCTGGACGGTTG-3' | 363 |
5 | 5'-CTTCTCCTTGGGGATAGTGCAT-3' | 5'-ACACTACAGCCACGAGCAGAG-3' | 358 |
6 | 5'-GCCTAGAGCTAGATGGGAATTTAAG -3' | 5'-GATTCCAGCCTCAGTTCAAACC-3' | 617 |
7 | 5'-CTTTAGAGCAGACAAGTGCTAGGAA- 3' | 5'-ACCATACCCATAACCCACTGACTC-3' | 373 |
8 | 5'-AGCATAAGGATCAGACCCAAGTGT-3' | 5'-TAACTCTGTGGCTTCTTGTCTCTC-3' | 400 |
9 | 5'-GTTAATTCTGGGACAGATGGCTTC-3' | 5'-CTCTCTTCACCCCTCCTCTTTG-3' | 238 |
10-1 | 5'-CCATCTCTGTGCAGGAAAAGTC-3' | 5'-AGCTGAGCTGCTTGGGTGATA-3' | 698 |
10-2 | 5'-ACTGACGGAGCCACAGACTC-3' | 5'-GGCTATCAACTTTCGCAGGA-3' | 668 |