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Table 1 CD4 exonic primer sets for DNA sequencing

From: Identification and characterization of two CD4 alleles in Microminipigs

Amplification exon Forward primer sequence Reverse primer sequence Length of PCR product size (bp)
2 5'-GTACCTGTGGGTGTCAGTTTAGAG-3' 5' -CTTACCCAGCACCAGATATTTTTC-3' 383
3 5'-CTCAGACTCAAACTGGGATGATTG -3' 5' -GATCCCAGAGTTTACTAGGAGCTG-3' 366
4 5'-AATGAGCAACTCAGATCAGAAGAGT-3' 5'-CTTATCCATCTCTGGACGGTTG-3' 363
5 5'-CTTCTCCTTGGGGATAGTGCAT-3' 5'-ACACTACAGCCACGAGCAGAG-3' 358
6 5'-GCCTAGAGCTAGATGGGAATTTAAG -3' 5'-GATTCCAGCCTCAGTTCAAACC-3' 617
7 5'-CTTTAGAGCAGACAAGTGCTAGGAA- 3' 5'-ACCATACCCATAACCCACTGACTC-3' 373
8 5'-AGCATAAGGATCAGACCCAAGTGT-3' 5'-TAACTCTGTGGCTTCTTGTCTCTC-3' 400
9 5'-GTTAATTCTGGGACAGATGGCTTC-3' 5'-CTCTCTTCACCCCTCCTCTTTG-3' 238
10-1 5'-CCATCTCTGTGCAGGAAAAGTC-3' 5'-AGCTGAGCTGCTTGGGTGATA-3' 698
10-2 5'-ACTGACGGAGCCACAGACTC-3' 5'-GGCTATCAACTTTCGCAGGA-3' 668