Fig. 5
From: Identification and characterization of two CD4 alleles in Microminipigs
Alignment of amino-acid sequences of CD4.A-FLAG and CD4.B-FLAG and their exon 8 deficiency forms. (.) indicates having identical sequence to CD4.A-FLAG. Arrow indicates the putative boundary of each exon. (*) indicates the stop codon. The regions of two extracellular Ig-like domains (Domain 3, 4), joining region (JR 3, 4), transmembrane (TM) domain, cytoplasmic domain and FLAG are also shown. Exon 8 deficiency forms gave rise to a stop codon at the N-terminus of TM domain as a result of a frameshift from the beginning of the exon 8 region, even though amino-acid sequences of their external CD4 domains in the spliced forms were identical to those of CD4.A-FLAG and CD4.B-FLAG. Thus, we used the constructs with complete sequences of CD4.A-FLAG and CD4.B-FLAG for the expression study in HeLa cells