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Table 1 Summary of immunohistochemical methodology

From: Pulmonary carcinoma with metastasis in a long-finned pilot whale (Globicephala melas)

Antibody Source Host Type Clone Antigen retrieval Dilution
Pancytokeratins Dakoa Mouse Monoclonal AE1/AE3 10 % pronaseb 1 in 100
CK 5 + 8 Euro-Diagnosticac Mouse Monoclonal RCK-102 10 % pronase 1 in 20
CK 8 + 18 Euro-Diagnostica Mouse Monoclonal NCL-5D3 Citrate bufferd 1 in 20
CK7 Dako Mouse Monoclonal OV-TL 12/30 Citrate buffer 1 in 50
CK 20 Dako Mouse Monoclonal Ks 20.8 Citrate buffer 1 in 25
Vimentin Dako Mouse Monoclonal Vim 3B4 Citrate buffer 1 in 100
  1. CK cytokeratin
  2. aDako, Glostrup, Denmark
  3. b10 % pronase, 10 min at room temperature
  4. cEuro-Diagnostica, Arnhem, The Netherlands
  5. dCitrate buffer, pH 6.0, 20 min at 95