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Table 1 Summary of immunohistochemical methodology

From: Pulmonary carcinoma with metastasis in a long-finned pilot whale (Globicephala melas)

Antibody

Source

Host

Type

Clone

Antigen retrieval

Dilution

Pancytokeratins

Dakoa

Mouse

Monoclonal

AE1/AE3

10 % pronaseb

1 in 100

CK 5 + 8

Euro-Diagnosticac

Mouse

Monoclonal

RCK-102

10 % pronase

1 in 20

CK 8 + 18

Euro-Diagnostica

Mouse

Monoclonal

NCL-5D3

Citrate bufferd

1 in 20

CK7

Dako

Mouse

Monoclonal

OV-TL 12/30

Citrate buffer

1 in 50

CK 20

Dako

Mouse

Monoclonal

Ks 20.8

Citrate buffer

1 in 25

Vimentin

Dako

Mouse

Monoclonal

Vim 3B4

Citrate buffer

1 in 100

  1. CK cytokeratin
  2. aDako, Glostrup, Denmark
  3. b10 % pronase, 10 min at room temperature
  4. cEuro-Diagnostica, Arnhem, The Netherlands
  5. dCitrate buffer, pH 6.0, 20 min at 95