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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Epidemiological and pathological study of feline morbillivirus infection in domestic cats in Japan

Fig. 5

Analysis of recombinant FmoPV-N protein. a Coomassie Brilliant Blue (CBB) staining and Immunoblot. Two major bands were stained with CBB (M; mock, FN; FmoPV-N) (I). A large band corresponded to the entire FmoPV-N with a molecular weight of 59 kDa (1), while other smaller band may correspond to a truncated FmoPV-N with a molecular weight of 40 kDa (2). FmoPV antibody positive cat serum (N028) reacted to both two bands (II). FmoPV antibody negative cat serum (N004) did not react to mock and FmoPV-N protein (III). b Purity of the recombinant FmoPV-Ab. An image of the stained gel was analyzed using ImageJ 1.50i and it was shown that amount of the entire FmoPV-N and the truncated protein were approximately 21 and 72 %, respectively. The purity was estimated to be 93 %

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