Development of a novel lateral flow assay for detection of African swine fever in blood

BMC Veterinary Research

Table 2 Description of the false negative (FN) samples using the LFA by the analysis of experimental samples collected from animals infected with ASFV genotype II and IX viruses

ASFV Strain	ID PIG	Days post infection	UPL-PCR CT value	OIE real-time PCR CT value	Virus isolation (TITER _HAD50/ml)	Ag-ELISA (INGENASA).
LT14/1490	L10	13	30.88	30.39	3,16 × 10³	FN*
LT14/1490	L10	17	17.61	16.49	6,81 × 10⁵	POS
LT14/1490	L11	13	26.43	27.41	3,16 × 10²	FN
LT14/1490	L12	13	36.67	37.21	NEGATIVE	FN
LT14/1490	L13	3	32.73	32.47	NEGATIVE	FN
LT14/1490	L14	17	33.04	34.79	NEGATIVE	FN
LT14/1490	L15	13	25.93	24.71	6,81 × 10⁴	FN
LT14/1490	L15	17	18.27	16.73	6,81E × 10⁶	FN
LT14/1490	L16	13	32.41	32.62	NEGATIVE	FN
LT14/1490	L17	3	37.46	37.45	NEGATIVE	FN
LT14/1490	L17	7	23.14	23.07	3,16 × 10⁵	POS
LT14/1490	L18	17	36.95	FN	NEGATIVE	FN
LT14/1490	L18	34	38.51	FN	NEGATIVE	FN
LT14/1490	L18	38	38.46	FN	NEGATIVE	FN
LT14/1490	L2	14	34.54	33.95	3,16E + 01	FN
LT14/1490	L3	3	32	33.04	NEGATIVE	FN
LT14/1490	L4	10	31.67	31.85	3,16E + 03	FN
LT14/1490	L6	14	27.2	26.91	3,16E + 08	FN
Ken06.Bus	CC6	D10	35.34	39.63	NEGATIVE	FN

ISSN: 1746-6148