Bile components and lecithin supplemented to plant based diets do not diminish diet related intestinal inflammation in Atlantic salmon

Kortner, Trond M.; Penn, Michael H.; Bjӧrkhem, Ingemar; Måsøval, Kjell; Krogdahl, Åshild

doi:10.1186/s12917-016-0819-0

Table 1 Diet formulation and chemical analysis

From: Bile components and lecithin supplemented to plant based diets do not diminish diet related intestinal inflammation in Atlantic salmon

	HFM	LFM	LFM +	LFM +	LFM +	LFM +	LFM +	HPS	HPS +	HPS +
			Tauro-cholate	Bovine bile salt	Taurine	Lecithin	Suppl Mix		Bovine bile salt	Suppl Mix
Ingredient (g 100 g ⁻¹ )
SA SP Sara Rousing^a	15.00	5.00	5.00	5.00	5.00	5.00	5.00	7.50	7.50	7.50
Nordic LT 94 fishmeal^b	15.00	5.00	5.00	5.00	5.00	5.00	5.00	7.50	7.50	7.50
Soya HP 48^c								20.00	20.00	20.00
Soya 60 % (SPC)^d	10.00	19.03	19.03	19.03	19.03	19.03	19.03	3.52	3.52	3.52
Corn Gluten^e	5.34	15.00	15.00	15.00	15.00	15.00	15.00	10.19	10.19	10.19
Pea Protein 50^f	6.00	13.00	13.00	13.00	13.00	13.00	13.00	0.40	0.40	0.40
Dehulled Beans^g	14.58	14.00	14.00	14.00	14.00	14.00	14.00	14.00	14.00	14.00
Sunflower expeller^h	10.00
Wheat Glutenⁱ		1.97	1.97	1.97	1.97	1.97	1.97	10.00	10.00	10.00
Fishoil (Standard)^j	7.45	7.67	7.67	7.67	7.67	7.67	7.67	7.82	7.82	7.82
Rapeseed oil^k	17.09	17.60	17.60	17.60	17.60	17.60	17.60	17.94	17.94	17.94
Sodium taurocholate^l			1.80
Bovine bile salt^l				1.80			1.80		1.80	1.80
Cholesterol^l							1.50			1.50
Taurine^l					0.40
Lecithin^l						1.50	0.40			0.40
MCP	0.70	1.82	1.82	1.82	1.82	1.82	1.82	1.56	1.56	1.56
Lysine	0.20	0.81	0.81	0.81	0.81	0.81	0.81	0.98	0.98	0.98
Methionine	0.11	0.32	0.32	0.32	0.32	0.32	0.32	0.25	0.25	0.25
Threonine		0.11	0.11	0.11	0.11	0.11	0.11	0.14	0.14	0.14
Barox (antioxidant)	0.03	0.03	0.03	0.03	0.03	0.03	0.03	0.03	0.03	0.03
Vitamin-Mineral mix^m	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30
Yttriumⁿ	0.05	0.05	0.05	0.05	0.05	0.05	0.05	0.05	0.05	0.05
Lucantin Pink CWD 10 %	0.04	0.04	0.04	0.04	0.04	0.04	0.04	0.04	0.04	0.04
Chemical analysis ^o
Dry matter (%)	95.0	95.0	95.0	95.0	95.0	95.0	95.0	95.0	95.0	95.0
Ash (%)	7.0	5.0	5.0	5.0	5.0	5.0	5.0	5.2	5.2	5.2
Fat (%)	29.8	29.2	29.2	29.2	29.2	29.2	29.2	29.6	29.6	29.6
Protein (%)	40.8	40.4	40.4	40.4	40.4	40.4	40.4	41.4	41.4	41.4
D Protein (%)	36.0	36.0	36.0	36.0	36.0	36.0	36.0	36.0	36.0	36.0
DE (MJ/kg)	20.3	20.3	20.3	20.3	20.3	20.3	20.3	20.3	20.3	20.3
GE (MJ/kg)	24.4	24.4	24.4	24.4	24.4	24.4	24.4	24.6	24.6	24.6
Cholesterol (g/kg)	2.1	0.8	0.7	0.6	0.7	0.7	19.1	1.0	1.0	17.8
Bile salts (g/kg)	0.3	0.2	16.3	n/a	n/a	n/a	11.0	n/a	10.58	n/a

^aSuperprime, supplied by Köster Marine Proteins GmbH, Hamburg, Germany
^bSupplied by Norsildmel AS, Bergen, Norway
^cSupplied by Scan Mills, Germany
^dSupplied by Selecta S/A, Av. Jamel Ceilio, 2496 – 12th region, Goiania, Brazil
^eSupplied by Cargil Nordic, SAS van Gent, Holland
^fSupplied by DLG Food Grain, Roslev, Denmark
^jSupplied by HC Handelscenter, Skibby, Denmark
^hSupplied by DLA agro, Denmark
ⁱSupplied by Roquette, Beinheim, France
^jSupplied by FF Skagen, Skagen, Denmark
^kSupplied by Emmelev, Otterup, Denmark
^lSupplied by Sigma-Aldrich, Broendby, Denmark
^mSupplied to meet requirements. Composition is intellectual property of BioMar AS
ⁿInert marker for the evaluation of nutrient digestibility
^oComplete chemical analysis was conducted only for the basal diets (HFM, LFM, HPS). Cholesterol content was analyzed in all diets, bile salt content was analyzed in five diets as shown in table

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