Fig. 3

The curve of virus replication after culture with QdNOs and its main metabolites (mean ± SD, n = 4). PK-15 cells were incubated with CSFV for 2 h, and then fresh medium containing the compounds (A: CYA, QCT, and MEQ; B: Cy1, Cy2, and Cy 10; C: Q2; D: M1, M4, M5, and M6) was added. The cell culture supernatants and CSFV-infected PK-15 cells were collected at 12 h intervals. After RNA isolation and cDNA synthesis, the samples were subjected to the real-time PCR to detect the copy number. The LogConcentration in the Y-axis indicates the denary logarithm of the copy number of CSFV per microliter. BK, blank control