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Table 1 Haematological parameters of Case 1 and Case 2 measured during clinical EEHV-1A infection

From: Surviving and fatal Elephant Endotheliotropic Herpesvirus-1A infections in juvenile Asian elephants – lessons learned and recommendations on anti-herpesviral therapy

A

Days

WBC (*10e9/L)

Neutrophils (*10e9/L)

Lymphocytes (*10e9/L)

Monocytes (*10e9/L)

Eosinophils (*10e9/L)

Basophils (*10e9/L)

   

7

22.85

7.77

8.91

5.94 (26 %)

0.23

0

   

23

13.69

5.75

2.87

6.16 (45 %)

0

0

   

213

15.91

4.61

5.73

5.25 (33 %)

0.32

0

   

Physiological mean

(% of WBC)

15.51

3.77 (25.5 %)

5.02

(23.65 %)

6.46

(50 %)

0.24

(1.55 %)

0.02

(0.10 %)

   

Days

RBC (*10e12/L)

HGB (g/dL)

HCT (%)

MCV (fL)

MCH (pg)

MCHV (g/dL)

RDW

(%)

PLT (*10e9/L)

PCV (%)

7

3.54

13.4

37.7

107

37.8

35.5

14.3

470

36

23

5.06

19.2

55.1

109

37.9

34.8

14.5

968

55

213

3.62

13.3

39.2

108

36.8

33.9

13.9

996

37

Physiological (mean)

3.55

14.28

40.60

114.25

40.19

35.17

14.33

617.65

40.80

B

Days

EEHV-1A

(vgc/ml)

WBC

(*10e9/L)

Neutrophils (*10e9/L)

Lymphocytes (*10e9/L)

Monocytes (*10e9/L)

Eosinophils (*10e9/L)

Basophils (*10e9/L)

  

-33

5.0 × 102

23.08

4.62

5.77

12.46 (54 %)

0

0

  

-29

ND

25.34

4.56

7.6

13.18 (52) %

0

0

  

-5

1.2 × 105

6.18

3.46

1.85

0.8 (13 %)

0.06

0

  

-3

2.7 × 106

7.35

5.22

1.32

0.81 (11 %)

0

0

  

0

7.2 × 106

13.25

8.22

2.65

2.25 (17 %)

0.13

0

  

1

1.9 × 107

12.925

7.565

2

2.975 (23 %)

0

0.195

  

Physiological mean (% of WBC)

 

15.51

3.77 (25.5 %)

5.02 (23.65 %)

6.46 (50 %)

0.24 (1.55 %)

0.02 (0.10 %)

  

Days

RBC (*10e12/L)

HGB (g/dL)

HCT (%)

MCV (fL)

MCH (pg)

MCHV (g/dL)

RDW (%)

PLT (*10e9/L)

PCV

(%)

-33

3.65

14.6

43.2

118.3

39.9

33.7

13.9

950

42

-29

3.58

14.5

41.4

115.5

40.4

35

38.1

760

38

-5

3.48

14.2

41.8

120.4

41

34

14

560

37

-3

3.36

14.2

38.4

114.4

42.4

37.1

13.7

183

38

0

2.89

12.4

32.9

113.7

42.9

37.8

13.8

98

34

1

3.07

12.95

35.05

114.2

42.2

36.9

13.6

13

36.5

Physiological mean

3.55

14.28

40.60

114.25

40.19

35.17

14.33

617.65

40.80

  1. The haematological parameters for Case 1 (A) were only available for Days 7, 23 and 213 following suspicion of clinical EEHV-1A infection. The haematological parameters and viral load for Case 2 (B) were measured on several occasions from 33 days prior to the observation of signs indicative of clinical EEHV-1A infection (Day 0) to the Day 1 when the elephant succumbed to the infection at midnight. Normal platelet range of live juveniles in the collection was established, during a period of seven months from 20 blood samples of the elephants, to be at 372-878*10e9/L (mean of 625*10e9/L). The rest of the physiological parameters were from 24 measurements from four individuals under eight years of age (age range at time of sampling: 1.5-7y). Parameter values with a substantial change are highlighted in bold. Percentages of monocytes in the white blood cells are also given in the table
  2. vgc/ml, viral genome copies per millilitre of EDTA whole blood; WBC, white blood cell count; RBC, red blood cell count; HGB, haemoglobin; HCT, haematocrit; MCV, mean cell volume; MCH, mean corpuscular haemoglobin; MCHV, mean corpuscular haemoglobin volume; RDW, red blood cell distribution width; PLT, platelet count; PCV, packed cell volume; ND, not detected