Table 1 Primer sequences for real-time PCR verification of microarray result
From: Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis
Gene | Forward primer (5'–3') | Revers primer (5'–3') | NCBI accession number |
|---|---|---|---|
PIAS1 | TGGAGTTGATGGATGCTTGAG | GGACACTGGAGATGCTTGAT | transcript variant X1-X2: XM_005638536.2 5 XM_535524.5 |
RORA | AAGGCTGCAAGGGCTTTTTC | CTGCGTACAAGCTGTCTCTT | transcript varian X1- X3: XM_014109378.1 XM_535503.6 XM_014117330.1 |
SH2B1 | CGTCCTCACTTTCAACTTCCA | GACACGACATAGCTGACAAGA | transcript variant X1-X8 XM_005621372.2 XM_005621371.2 XM_005621373.2 XM 005621374 XM_014114512.1 XM_014114513.1 XM_014114514.1 XM_014114515.1 |
GAPDH | GGAGAAAGCTGCCAAATATG | ACCAGGAAATGAGCTTGACA | NM_001003142.1 |
RPS19 | CCTTCCTCAAAAAGTCTGGG | GTTCTCATCGTAGGGAGCAAG | XM_533657.3 |