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Table 3 Bacterial strains and plasmids used in the present study

From: Iron-regulated gene ireA in avian pathogenic Escherichia coli participates in adhesion and stress-resistance

Strain or plasmid

Characteristics

Reference

Strain

 DE205B

O2:K1, NalR

[16, 19]

 DE205BΔireA

ireA deletion mutant strain

This study

 DE205BCΔireA

ireA complementary strain

This study

Plasmid

 pKD46

Amp, express λ red recombinase

[29]

 pKD4

kan, template plasmid

[29]

 pSTV28

Cm, expression using lac promotor

TAKARA

 pCP20

Cm, Amp, yeast Flp recombinase gene, FLP

[29]

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BMC Veterinary Research

ISSN: 1746-6148

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