Sarcoptic mange in the Scandinavian wolf Canis lupus population

Fuchs, Boris; Zimmermann, Barbara; Wabakken, Petter; Bornstein, Set; Månsson, Johan; Evans, Alina L.; Liberg, Olof; Sand, Håkan; Kindberg, Jonas; Ågren, Erik O.; Arnemo, Jon M.

doi:10.1186/s12917-016-0780-y

BMC Veterinary Research

Table 2 Results from ELISA and Western Blot analysis

From: Sarcoptic mange in the Scandinavian wolf Canis lupus population

ID Wolf	Sampling date	Sex	Age class	OD MSA – 1	OD Crude	Result ELISAs	Western Blots
9802	15.12.1998	Male	Adult	0.71	0.83	++	++
9803	15.12.1998	Female	Adult	0.73	0.91	++	+
9808	27.01.2000	Male	Adult	0.11	0.32	−+	--
0001	28.01.2000	Male	Adult	1.54	2.18	++	++
0002	28.01.2000	Female	Adult	0.11	0.09	--	-
0003	28.01.2000	Female	Juvenile	0.05	0.07	--	-
0104	10.02.2001	Female	Adult	1.14	1.20	++	++
0105	10.02.2001	Male	Adult	1.66	1.96	++	++
0105	05.02.2003	Male	Adult	0.76	0.73	++	+
0109	12.02.2001	Male	Adult	0.63	0.11	+−	-
0109	08.12.2001	Male	Adult	0.34	0.13	--	-
0106	12.02.2001	Female	Juvenile	0.11	0.14	--	-
0111	21.12.2001	Female	Adult	0.20	0.30	-?	+
0203	13.01.2002	Female	Adult	0.17	0.24	-?	-
0208	26.01.2002	Male	Adult	0.41	0.15	?-	-
0208	06.03.2003	Male	Adult	1.32	1.30	++	+
0215	01.02.2002	Male	Adult	0.20	0.25	-?	-
0214	05.03.2003	Male	Adult	0.70	0.92	++	+
0214	21.01.2004	Male	Adult	0.41	0.65	?+	+
0307	06.03.2003	Female	Adult	0.15	0.23	-?	-
0009	23.01.2004	Male	Adult	0.36	0.13	?-	-
0504	14.03.2005	Male	Adult	0.40	0.42	?+	+
0506	16.03.2005	Male	Adult	0.56	0.19	+−	?+
0506	09.03.2007	Male	Adult	0.43	0.07	?-	-
0507	16.03.2005	Female	Juvenile	0.26	0.15	--	-
0507	08.02.2008	Female	Adult	0.65	0.13	+−	-
0601	27.01.2006	Female	Adult	0.44	0.37	?+	-
0606	01.02.2006	Male	Adult	0.22	0.36	−+	+
0606	08.03.2007	Male	Adult	0.12	0.20	--
0611	13.02.2006	Male	Adult	0.22	0.25	-?	+
0611	11.03.2007	Male	Adult	0.43	0.21	??	-
0704	07.03.2007	Female	Adult	0.67	0.70	++	+
0913	30.01.2009	Male	Juvenile	0.10	0.35	−+	-
0916	11.02.2009	Male	Adult	1.50	0.40	++	+
0918	12.02.2009	Male	Adult	1.01	0.80	++	+
0918	11.02.2010	Male	Adult	0.23	0.19	--
1004	10.02.2010	Female	Juvenile	0.18	0.22	-?	-
1114	21.03.2011	Female	Adult	0.73	0.39	++	+
1114	11.12.2011	Female	Adult	0.98	0.37	++	-
1114	06.02.2012	Female	Adult	0.81	0.35	++	?-
1114	14.03.2013	Female	Adult	1.19	0.37	++	+
1202	16.12.2011	Male	Adult	1.54	0.98	++	+

OD MSA-1: Relative optical densities using the MSA-1 antigen in the ELISA, cut off are 0.35 (doubtful) and 0.499 (seropositive) respectively. OD Crude: Relative optical densities using the crude antigen in the ELISA, cut off 0.2 (doubtful) and 0.299 (seropositive). Result ELISAs are corresponding to the OD values and decide between: + (positive), − (negative) and ? (doubtful). Shown are all samples with either positive or doubtful results from one of the ELISA and five random selected with negative results

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ISSN: 1746-6148

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