Identification of the two KIT isoforms and their expression status in canine hemangiosarcomas

BMC Veterinary Research

Table 2 Sequences and locations of c-kit primers used in this study

Designation	Sequence (5′-3′)	Primer location
1F	CGATGAGAGGCGCTCGC	27–34
1R	GGCGTAACACA TGAACACTCCAG	891–913
2F	GCTGGCATCATGGTGACTTC	819–838
2R	CATGGGTTTCTGTAGATACTTGTAGG	1668–1693
3F	CACACCTTTGCTGATTGGCT	1591–1610
3R	GATTCGACCATGAGTAAGGAGG	2419–2440
4F	GGGTATGGCATTCCTGGC	2359–2376
4R	GCTTCACACATCTTCGTGTACCA	2937–2959
AF	GCTCAGAGTCTATCGCAGCCACCG	3–26
AR	CTGCCTTCTCTGTGATCCATTCGTTG	271–296
BF	GCTGTCCAAGAAATTCACCCTG	619–640
BR	ATATTACTTTCATTGTCAGACTTGGG	1124–1149
CF	AAAACTCGTCTCTGTCACCGTCTG	1422–1445
CR	GATCTCCTCAACAACCTTCCACTG	1691–1714
DF	AAATCAGAGTTAATAGTCAGTGTCGG	133–158
DR	TTTATCCACATCGAGTCCACG	729–749
EX9F	CAACAATGTAGGCAGGAGTTCTG	1495–1517
EX9R	CAGCAAAGGTGTGAACAGGG	1572–1591

F forward, R reverse
Annealing temperature dependent on combination of the primers: 55 °C for 1F and 1R, 56 °C for 2F and 2R, 52 °C for 3F and 3R, 61 °C for AF and AR, 54 °C for BF and BR, 57 °C for CF and CR, 54 °C for DF and DR, 57 °C for AF and BR, 55 °C for P2F and CR, 56 °C for CF and P3R, and 54 °C for EX9F and EX9R

ISSN: 1746-6148