Table 1 Primary and secondary antibodies and antigen retrieval procedures used in IHC analysis of this study
From: Identification of the two KIT isoforms and their expression status in canine hemangiosarcomas
Time for incubation | |||||||
|---|---|---|---|---|---|---|---|
Antibody | pAb/mAb (clone) | Host | Source | Antigen retrieval (buffer, microwave, interval) | Primary Ab | Secondary Ab | DAB+ Chromagen |
von Willebrand factor | pAb | Rabbit anti human | Dako | Citrate buffer (0.01 M pH 6.0),700 W-10 min and 300W-10 min | 1:200, 30 min | 10 min | 1 min |
vimentin | mAb (V9) | Mouse anti human | Dako | TE buffer (pH 9.0), 700 W-10 min and 400W-10 min | 1:150, 1 h | 10 min | 10 min |
KIT | pAb | Rabbit anti human | Dako | TE buffer (pH 9.0),700 W-10 min and 400W-10 min | 1:400, 30 min | 10 min | 2 min |