Table 2 Genes and sequence of primers evaluated in the study
Targeted genes | Primer sequences (5'- > 3') | Product size (bps) |
|---|---|---|
GAPDH, glyceraldehyde-3-phosphate dehydrogenase 6 | Forward TCCCTGCTTCTACTGGTGCT Reverse TGACAAAGTGGTCGTTGAGG | 306 |
NFkβ, nuclear factor of kappa light polypeptide gene enhancer in B-cells-like 1 | Forward CGATTTCGATATGGCTGTGA Reverse CACCTTCTTCAGCTCCTTGG | 399 |
MMP 13, matrix metallopeptidase 13 (collagenase 3) 7 | Forward GCATTCAAAAAGGCCTTCAA Reverse GGAAGCACAAAGTGGCTTTT | 356 |
ADAMTS 4, metallopeptidase with thrombospondin type 1 motif 4 | Forward TGTCAGCTTGGTGGTGACTC Reverse GTTGAAGACATGGCCCAGTT | 322 |
COL1A1, collagen, type I, alpha 1 11 | Forward AGCCAGCAGATCGAGAACAT Reverse CTGGCCACCATACTCGAACT | 309 |
COL2A1, collagen, type II, alpha 1 6 | Forward primer ACGTCCAGATGACCTTCCTG Reverse primer GTCCACACCAAATTCCTGCT | 326 |
COMP, cartilage oligomeric matrix protein 21 | Forward CCACGTGAATACGGTCACAG Reverse TAGGAACCAGCGGTAGGATG | 301 |