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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Canine distemper virus isolated from a monkey efficiently replicates on Vero cells expressing non-human primate SLAM receptors but not human SLAM receptor

Fig. 2

Infection of Vero cells expressing dog SLAM protein with CDV from a deceased rhesus monkey. Microscopic evaluation of Vero/DogSLAM cells (a) and Vero cells (b) following inoculation with Monkey-BJ01-DV at a MOI of 0.01. Inoculation of Vero/DogSLAM cells resulted in obvious CPE. Detection of CDV antigens in Vero cells expressing the dog SLAM protein (c) and untransfected Vero cells (d) 64 h following inoculation with Monkey-BJ01-CDV at a MOI of 0.01. Cells were fixed and probed with a mouse monoclonal anti-CDV nucleoprotein antibody. A FITC-conjugated goat anti-mouse secondary antibody was used for detection

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