Fig. 1From: Canine distemper virus isolated from a monkey efficiently replicates on Vero cells expressing non-human primate SLAM receptors but not human SLAM receptorStable expression of dog, monkey and human SLAM protein on Vero cells. Vero cells were transfected with expression plasmids to mediate expression of HA epitope-tagged SLAM proteins from dog, monkey, and human and selected with G418. The control Vero cells were transfected by empty vector. The resulting cell lines were named Vero/DogSLAM,Vero/MonkeySLAM and Vero/HumanSLAM. Anti-influenza virus HA epitope MAb was used to confirm surface expression of each version of the SLAM protein by flow cytometryBack to article page