Skip to main content

Table 2 The primer sequences of target and internal reference genes used in qPCR

From: Rumen-derived lipopolysaccharide enhances the expression of lingual antimicrobial peptide in mammary glands of dairy cows fed a high-concentrate diet

Gene

Forward primer

Reverse primer

GenBank accession

PCR products

LAP

AGGCTCCATCACCTGCTCCTT

CCTGCAGCATTTTACTTGGGCT

AY911374.1

183 bp

BNBD5

CTGGATCACCTGCTCCTCGT

GGTGCCAATCTGTCTCATGTTG

NM_001130761.1

150 bp

IL-1β

GGCCAAAGTCCCTGACCTCT

CTGCCACCATCACCACATTC

NM_174093.1

167 bp

IL-6

GGAGGAAAAGGACGGATGCT

GGTCAGTGTTTGTGGCTGGA

NM_173923.2

227 bp

IL-8

CCTCTTGTTCAATATGACTTCCA

GGCCCACTCAATAACTCTC

NM_173925.2

170 bp

TNF-α

CTTCTGCCTGCTGCACTTCG

GAGTTGATGTCGGCTACAACG

AF_348421.1

156 bp

NF-kB

ATACGTCGGCCGTGTCTAT

GGAACTGTGATCCGTGTAG

NM_001045868.1

129 bp

GAPDH

GGGTCATCATCTCTGCACCT

GGTCATAAGTCCCTCCACGA

XM_001252479

177 bp