Rumen-derived lipopolysaccharide enhances the expression of lingual antimicrobial peptide in mammary glands of dairy cows fed a high-concentrate diet

Table 1 Ingredients and nutrient composition of the experimental diets

Ingredients, % of DM	Percentage (%)of ingredients (dry matter)
Ingredients, % of DM	LCD¹	HCD¹
Corn silage	30.00	20.00
Alfalfa hay	30.00	20.00
Maize	22.78	33.6
Wheat bran	5.15	15
Soybean meal	9.81	9
Calcium phosphate dibasic	0.92	0.53
Powder	0	0.52
Salt	0.35	0.35
Premix²	1	1
Total	100	100
Forage:concentrate	6:4	4:6
Nutritional composition³
Net energy, MJ/kg	6.32	6.74
CP, %	16	16.2
EE, %	3.96	4.15
NDF, %	37.71	31.92
ADF, %	22.75	17.55
NFC, %	32.43	40.31
Ca, %	0.9	0.8
P, %	0.45	0.45

¹ LCD, low-concentrate diet; HCD, high-concentrate diet
² The premix contained VA,1,900kU/kg; VD, 250kU/kg; VE, 3000 mg/kg; niacin, 4000 mg/kg; Cu, 1200 mg/kg; Fe, 525 mg/kg; Zn, 13,000 mg/kg; Mn, 5500 mg/kg; I, 170 mg/kg; Co, 50 mg/kg; Se, 27 mg/kg
³ The calculated nutritional composition values

ISSN: 1746-6148