TY - JOUR AU - Jin, Di AU - Chang, Guangjun AU - Zhang, Kai AU - Guo, Junfei AU - Xu, Tianle AU - Shen, Xiangzhen PY - 2016 DA - 2016/06/27 TI - Rumen-derived lipopolysaccharide enhances the expression of lingual antimicrobial peptide in mammary glands of dairy cows fed a high-concentrate diet JO - BMC Veterinary Research SP - 128 VL - 12 IS - 1 AB - Long-term high-concentrate diet (HCD) feeding can cause subacute ruminal acidosis in cows and subsequently trigger systemic inflammatory and immune responses. Therefore, we conducted the present study in which twelve lactating cows installed with ruminal fistula were randomly assigned to the HCD group (forage:concentrate = 4:6, n = 6) or the low-concentrate diet (LCD) group (forage:concentrate = 6:4, n = 6) and were fed for 20 weeks. Ruminal fluid, plasma and mammary gland tissue samples were collected at week 20 for analysing lipopolysaccharide (LPS), pro-inflammatory cytokines, and immune relevant gene expression. The aim of this study was to investigate the effect of rumen-derived LPS on lingual antimicrobial peptide (LAP) synthesis and immune responses in mammary glands of lactating cows fed a HCD. SN - 1746-6148 UR - https://doi.org/10.1186/s12917-016-0755-z DO - 10.1186/s12917-016-0755-z ID - Jin2016 ER -