Table 1 Primers used in this study

IRF5-F

IRF5-R

GCAAGTACCAGGAAGGAG

AAATCAGTTAAGGGCAACA

Homologous amplification

IRF5-3 F1

IRF5-3 F2

IRF5-5R1

IRF5-5R2

IRF5g1-F

IRF5g1-R

GTCACTCATTCTGTAGGCCCT

GCAGAGAACCAGGTTCATCC

TGTCCTTCGTGCCATCGTAGTG

GTTTAGGGCACAGCGGAGATT

CAGCACCATGAGTGGTCAACCACG

AATATAGAGTTCTCCTCCTCCAG

First round 3′-RACE PCR

Nested 3′-RACE PCR

First round 5′-RACE PCR

Nested 5′-RACE PCR

For the first intron

IRF5g2-F

IRF5g2-R

AGGACTGCACTGGCTCAATCAAGA

ATCTACTCCTTCCTGATACTTGCCT

For the second intron

IRF5g3-F

IRF5g3-R

ATGGAAAGCCAATCTCCGCTGTG

GTAACATGAGCGGACTGCTCAGC

For the third, fourth and fifth intron

IRF5g4-F

IRF5g4-R

AGTGCAATCAAAGTTGAGCAGGCA

ATGATCGGTATAGAACCTCTGCTT

For the sixth intron

IRF5g5-F

IRF5g5-R

ACGCCATCAGGCTGTGCCAGTGTA

CAATGATGAGCTTTTTCTCCTTG

For the seventh intron

IRF5g6-F

IRF5g6-R

GCTTCGGTGAGGACTGGCCAGACA

ACTCTGCAGAAGTCTGTGGAGCT

For the eighth intron

IRF5-rF

IRF5-rR

CCTGGGCTCAGAACATCCACTAAC

AGTATGGCATCATAGAGGGCACCT

Real-time PCR

TNFα-F

TNFα-R

ACAGGTGATGGTGTCGAGGAGGA

TCTGAGACTTGTTGAGCGTGAAG

Real-time PCR

JF957372

S11-F

S11-R

CCGTGGGTGACATCGTTACA

TCAGGACATTGAACCTCACTGTCT

Real-time PCR

AB012087

ISG15-F

GTGAGCGGTGAAGCCACAGTTG

Real-time PCR

KP115358

ISG15-R

GCGAACCGTTATCGGCAGACAG

PKR-F

AGGCTTGATCCACAGAGACCTGAA

Real-time PCR

JX516101

PKR-R

CGTTCCAGAAGTTGCACGTCATTG