Fig. 2From: Redundant contribution of a Transient Receptor Potential cation channel Member 1 exon 11 single nucleotide polymorphism to equine congenital stationary night blindnessTissue-specific Nova-1 expression. RNA from retina (lanes 1 & 5), pigmented (lanes 2 & 6) and unpigmented skin (lanes 3 & 7) from a normal horse was reverse-transcribed using equal amounts of cDNA template in 30 cycles of PCR. PCR products were separated on agarose gel electrophoresis and identified by fluorescence with ethidium bromide. Lanes 1–3 were amplified with intron-spanning primers E c Nova-1 F16 – R224, product length = 209 bp. Lanes 5–7 were amplified with E c Nova-1 F1024 – R1454 primers spanning the stop codon, ;product length = 431 bpBack to article page