Release kinetics of tumor necrosis factor-α and interleukin-1 receptor antagonist in the equine whole blood

BMC Veterinary Research

Table 2 Maximal IL-1Ra production after stimulation with LPS, PCPwL or erTNF- α

BC (%)	LPS		PCPwL	erTNF-α
BC (%)	48 h	72 h	48 h	48 h	72 h
10 %	5643 ± 1520	5809 ± 1426	8045 ± 2965	5083 ± 2364	5120 ± 2323
20 %	11368 ± 1713	8502 ± 1667	11451 ± 1620	11167 ± 994	8829 ± 178
50 %	26375 ± 4842	21260 ± 7332	25982	>40000	27529 ± 4010

Supernatant IL-1Ra production (pg/ml) was compared between different whole blood volumes after stimulation with LPS, PCPwL or erTNF-α. Maximal concentrations were obtained after the start of stimulation at various time points until 72 h. Data represent means ± SEM of three different horses. BC, blood concentration

ISSN: 1746-6148