Fig. 1From: Toxin-neutralizing antibodies protect against Clostridium perfringens-induced necrosis in an intestinal loop model for bovine necrohemorrhagic enteritisWestern blot analysis of the immune sera. a SDS-PAGE of the C. perfringens toxin preparation after Coomassie staining. b-d Representative Western blots showing the immunoreactivity towards crude C. perfringens supernatants (lane 1) and the C. perfringens toxin preparation (lane 2). The immune sera from calves vaccinated with native toxins (b) and formaldehyde inactivated, L-lysine protected C. perfringens toxins (c) detect only two proteins, whereas the commercial formaldehyde inactivated multivalent clostridial vaccine (d) reacts with multiple proteins. The blots shown are representative pictures of one out of three experimentsBack to article page