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Table 1 Results from the PMMS-phage assay, faecal culture, faecal PCR and serum ELISA for sub-clinical, experimentally exposed cattle to MAP

From: Evaluation of the limitations and methods to improve rapid phage-based detection of viable Mycobacterium avium subsp. paratuberculosis in the blood of experimentally infected cattle

    Results
Sample Breed MAP exposure statusa PMMS-Phage assayb Faecal culture Faecal PCRc Serum Ab ELISA (%)d
1 Holstein Control - (0) - - 1.00
2 Holstein Control - (0) - - 29.34
3 Holstein Control - (2) - - 3.85
4 Holstein Control - (0) - - 5.92
5 Holstein Control - (0) - - 5.42
6 Holstein Control - (0) - - 3.14
7 Holstein Control - (0) - - 17.49
8 Holstein Control - (0) - - 10.49
9 Red/Holstein Control - (0) - - 3.50
10 Red/Holstein Control - (3) - - 4.57
11 Holstein Inoculated - (0) - - 2.57
12 Holstein Inoculated + (2) - * 3.28
13 Holstein Inoculated - (0) - - 5.00
14 Holstein Inoculated #e #e #e #e
15 Holstein Inoculated - (5) - - 14.49
16 Holstein Inoculated + (5) - - 3.28
17 Holstein Inoculated - (0) + + 48.68
18 Holstein Inoculated + (2) - - 8.57
19 Holstein Inoculated - (0) - - 6.21
20 Holstein Inoculated + (2) - - 35.62
21 Holstein Inoculated - (2) - - 12.63
22 Holstein Inoculated - (0) - - 3.50
23 Holstein Inoculated - (0) + + 118.77
24 Holstein Inoculated - (0) - - 6.50
25 Holstein Inoculated - (0) - * 4.64
26 Red/Holstein Inoculated + (4) - - 5.21
27 Holstein Inoculated + (5) - * 41.11
28 Holstein Inoculated - (0) - - 2.00
29 Red/Holstein Inoculated + (3) - - 13.28
30 Red/Holstein Inoculated - (0) - * 1.50
  1. aInoculated – animals experimentally exposed to MAP; Control – animals not
  2. exposed to MAP
  3. b+/- indicates result of combined PMMS-phage –PCR assay. Plaque numbers
  4. for each sample given in brackets
  5. c* indicates MAP DNA was detected but the quantity detected was below the cut-point for a positive result [16]
  6. dSerum Ab ELISA (IDEXX); Positive value > 55 %, suspected value 45–55 %
  7. e# Animal 14 was culled due to other illness unrelated to Johne’s disease before sample collection