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Table 1 Results from the PMMS-phage assay, faecal culture, faecal PCR and serum ELISA for sub-clinical, experimentally exposed cattle to MAP

From: Evaluation of the limitations and methods to improve rapid phage-based detection of viable Mycobacterium avium subsp. paratuberculosis in the blood of experimentally infected cattle

   

Results

Sample

Breed

MAP exposure statusa

PMMS-Phage assayb

Faecal culture

Faecal PCRc

Serum Ab ELISA (%)d

1

Holstein

Control

- (0)

-

-

1.00

2

Holstein

Control

- (0)

-

-

29.34

3

Holstein

Control

- (2)

-

-

3.85

4

Holstein

Control

- (0)

-

-

5.92

5

Holstein

Control

- (0)

-

-

5.42

6

Holstein

Control

- (0)

-

-

3.14

7

Holstein

Control

- (0)

-

-

17.49

8

Holstein

Control

- (0)

-

-

10.49

9

Red/Holstein

Control

- (0)

-

-

3.50

10

Red/Holstein

Control

- (3)

-

-

4.57

11

Holstein

Inoculated

- (0)

-

-

2.57

12

Holstein

Inoculated

+ (2)

-

*

3.28

13

Holstein

Inoculated

- (0)

-

-

5.00

14

Holstein

Inoculated

#e

#e

#e

#e

15

Holstein

Inoculated

- (5)

-

-

14.49

16

Holstein

Inoculated

+ (5)

-

-

3.28

17

Holstein

Inoculated

- (0)

+

+

48.68

18

Holstein

Inoculated

+ (2)

-

-

8.57

19

Holstein

Inoculated

- (0)

-

-

6.21

20

Holstein

Inoculated

+ (2)

-

-

35.62

21

Holstein

Inoculated

- (2)

-

-

12.63

22

Holstein

Inoculated

- (0)

-

-

3.50

23

Holstein

Inoculated

- (0)

+

+

118.77

24

Holstein

Inoculated

- (0)

-

-

6.50

25

Holstein

Inoculated

- (0)

-

*

4.64

26

Red/Holstein

Inoculated

+ (4)

-

-

5.21

27

Holstein

Inoculated

+ (5)

-

*

41.11

28

Holstein

Inoculated

- (0)

-

-

2.00

29

Red/Holstein

Inoculated

+ (3)

-

-

13.28

30

Red/Holstein

Inoculated

- (0)

-

*

1.50

  1. aInoculated – animals experimentally exposed to MAP; Control – animals not
  2. exposed to MAP
  3. b+/- indicates result of combined PMMS-phage –PCR assay. Plaque numbers
  4. for each sample given in brackets
  5. c* indicates MAP DNA was detected but the quantity detected was below the cut-point for a positive result [16]
  6. dSerum Ab ELISA (IDEXX); Positive value > 55 %, suspected value 45–55 %
  7. e# Animal 14 was culled due to other illness unrelated to Johne’s disease before sample collection