Association of anticardiolipin, antiphosphatidylserine, anti-β2 glycoprotein I, and antiphosphatidylcholine autoantibodies with canine immune thrombocytopenia

Table 1 Average IgG levels of antiphospholipid antibodies (aPL) among different groups of dogs

aPL subtype	I_A ^a	I_B	I_C	II^d	III^e	98^th/99^thpercentile cut-off GPL
	(n = 20)	(n = 18)	(n = 26)	(n = 30)	(n = 80)
	GPL^b (95 % CI)^c	GPL (95 % CI)	GPL (95 % CI)	GPL (95 % CI)	GPL (95 % CI)
aPhL	95.9 (79.3 – 112.5)	116.7 (99.4 – 134.0)	130.9 (105.2 – 156.6)	66.8 (56.5 – 77.0)	7.4 (5.8 – 9.1)	23.1/25.4
aβ₂GPI	65.7 (50.6 – 80.7)	40.8 (30.2 – 51.4)	64.9 (44.6 – 85.2)	40.4 (29.0 – 51.8)	6.1 (4.0 – 8.3)	26.7/30.4
aCL	38.8 (28.8 – 48.8)	49.6 (34.3 – 65.0)	58.9 (43.8 – 73.9)	18.3 (11.9 – 24.8)	0.8 (0.2 – 1.3)	5.6/7.1
aPI	18.5 (12.6 – 24.3)	24.4 (19.2 – 29.7)	17.1 (10.8 – 23.4)	15.6 (11.4 – 19.8)	4.2 (2.4 – 5.9)	20.3/23.6
aPC	21.0 (14.5 – 27.4)	17.4 (12.7 – 22.1)	17.5 (12.6 – 22.3)	11.8 (8.4 – 15.2)	3.2 (2 – 4.4)	15.7/17.5
aPS	16.8 (11.0 – 22.5)	18.0 (12.9 – 23.0)	13.5 (8.4 – 18.6)	11.2 (6.3 – 16.2)	0.8 (0.2 – 1.4)	5.6/7.3

^aSubgroups of Group I (thrombocytopenia): I_A (immune thrombocytopenia, aPLT⁺, platelet < 30 × 10³/uL), I_B (severe thrombocytopenia, aPLT^-, platelet < 30 × 10³/uL), and I_C (less severe thrombocytopenia, platelet 30 – 80 × 10³/uL). ^bConcentrations (μg/mL of IgG, GPL) were converted directly from the OD readings based on the equation shown in Fig. 1a. One GPL unit is defined as the binding activity of 1 μg/mL of IgG. ^c95% confidence interval. ^dOutpatient dogs without thrombocytopenia (Group II). ^eHealthy dogs (Group III)

ISSN: 1746-6148