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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Association of anticardiolipin, antiphosphatidylserine, anti-β2 glycoprotein I, and antiphosphatidylcholine autoantibodies with canine immune thrombocytopenia

Fig. 1

Dog IgG and human aPhL IgG standard curves. A sandwich ELISA was performed to establish a calibration curve for dog IgG (panel a). Rabbit anti-dog IgG F(ab)2 fragment antibodies were coated onto the ELISA plate. Different concentrations (200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.125 ng/mL) of dog IgG were added into each well. Peroxidase conjugated-rabbit anti-dog IgG Fc fragment antibody was used to detect dog IgG. OD = 0.0109 × concentration + 0.0787. R2 = 0.9726. To obtain a human aPhL IgG standard curve, aPhL IgG reference calibrators (200, 100, 50, 25, 12.5, and 6.25 μg/mL) available in a human APhL IgG ELISA kit were used (panel b). OD = 0.0086 × concentration + 0.0482. R2 = 0.9961. The OD (450 nm) values are shown as means ± standard error (SE)

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BMC Veterinary Research

ISSN: 1746-6148

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