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Table 1 Synthesized siRNAs sequences targeting the regions of PRRSV nsp11 and the primers of ORF-7 for RT-PCR

From: Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells

Gene of target

Name of siRNA

Location(bp)

Sequence (5’-3’)

nsp 11

nsp11 siRNA 1

244-262

CGTGTCATACTATCTCACA

nsp 11

nsp11 siRNA 2

526-544

CACACTGACAGATGTGTAC

nsp 11

nsp11 siRNA 3

409-427

CACTACCGTTGGAGGATGT

Negative control

Control siRNA

 

CCTACGCCACCAATTTCGT

ORF-7 For

  

AAACCAGTCCAGAGGCAAGG

ORF-7 Rev

  

GCAAACTAAACTCCACAGTGTAA

GAPDH For

  

TGACAACAGCCTCAAGATCG

GAPDH Rev

  

GTCTTCTGGGTGGCAGTGAT

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BMC Veterinary Research

ISSN: 1746-6148

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