Fig. 3

siRNAs targeting nsp11 could efficiently reduce the titers of PRRSV. a 293T cells grown in 24-well plates were transfected with pcDNA3.1-GFP-nsp11(800 ng/well) or pcDNA3.1-GFP (800 ng/well) and nsp11 siRNA 1 (100 nM), nsp11 siRNA 2 (100 nM) or control siRNA (100 nM), then 36 h later, the cells for collected for the western blot. b MARC-145 cells cultured in 24-well plates were transfected with nsp11 siRNA 1 (100 nM), nsp11 siRNA 2 (100 nM) or control siRNA (100 nM). And 6 h later, the cells were infected with PRRSV at an MOI of 0.1 or mock infected, and 24 h later, the cells were lysed by freezing and thawing three times, then the viral titers were measured by TCID50. c MARC-145 cells grown in 24-well plates were transfected with nsp11 siRNA 1 (100 nM), nsp11 siRNA 2 (100 nM) or control siRNA (100 nM). And 6 h later, the cells were infected with PRRSV at an MOI of 0.1 or mock infected, and 24 h later, the cells were collected and the viral RNA levels were measured by RT-PCR. Data represented means of three replicates, and experiments were repeated three times. Error bars represented the standard deviations. *: P <0.05 compared with the results in control. MOI, multiplicity of infection