Fig. 2

siRNAs targeting nsp11 could inhibit the expression of GFP- nsp11 and didn’t influence on the expression of GFP in 293T cells or in MARC-145 cells. 293T cells (a) or MARC-145 cells (b) cultured in 24-well plates were co-transfected with pcDNA 3.1-GFP-nsp11(800 ng/well) or pcDNA3.1-GFP (800 ng/well) and nsp11 siRNA 1 (100 nM), nsp11 siRNA 2 (100 nM), nsp11 siRNA 3(100 nM) or control siRNA (100 nM). And 24 h later, the cells were analyzed by fluorescence microscopy (50×). Data represented means of three replicates, and experiments were repeated three times