Fig. 2From: Development of monoclonal antibodies and serological assays including indirect ELISA and fluorescent microsphere immunoassays for diagnosis of porcine deltacoronavirusAntibody capture efficacy comparison between refolded vs linear PDCoV-NP. An ELISA antibody capture titration assay was employed to compare the ability of refolded vs. linear antigen to capture anti-PDCoV-NP specific antibody in swine serum. Wells of a 96-well microtiter plate were coated with decreasing concentrations of either linearly expressed or refolded PDCoV-NP antigen. Refolded antigen demonstrated greater dynamic range of the assay and capture efficacy of swine antibodyBack to article page