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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Development of monoclonal antibodies and serological assays including indirect ELISA and fluorescent microsphere immunoassays for diagnosis of porcine deltacoronavirus

Fig. 1

Purification and antigen specificity of PDCoV-NP antibody capture antigen. a Coomassie blue staining of E. coli expressed and purified PDCoV-NP antigen used to coat ELISA microtiter plates and FMIA microspheres. Molecular weight ladder MW and PDCoV-NP (41 kDa). b Western blot showing antigen specificity of recombinant, refolded PDCoV-NP/polyhistidine fusion protein probed with mAb SD-55-197 (Lane 1). Lane 2 was loaded with semi-purified, concentrated PDCoV strain HKU15/Michigan/8977/2014 then probed with mAb SD-55-197

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