Table 3 Primers used for reverse transcriptase-polymerase chain reaction of immortalized canine oral fibrosarcoma cell lines
From: Antiproliferative effects of masitinib and imatinib against canine oral fibrosarcoma in vitro
Target | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) | Spanned mRNA sequence | Product size (bp) |
|---|---|---|---|---|
PDGFRA | CCTCGATCCTTCCAAATGAA | GGTCACAAAAAGGCCACTGT | 357-523 | 167 |
PDGFRB | GTGGTATGGGAACGGTTGTC | GTGGGATCTGGCACAAAGAT | 228-421 | 194 |
KIT | CCCATTTAACCGAACGAGAA | TCTCCGTGATCTTCCTGCTT | 2016-2226 | 211 |
KDR | GATCGGTGAGAAATCCCTGA | CTGGAAGTCATCCACGTTT | 1266-1473 | 208 |