Fig. 6

PDGFR-β reduction following PDGFRB siRNA transfection. Effect of PDGFRB siRNA transfection on PDGFR-β expression in CoFSA and MBSa1 cells assessed via western blot. Reduced PDGFR-β levels are represented as decreased band intensity in the PDGFRB siRNA treated lanes for both cell lines. Cells were incubated with siRNA for 48 h, as described in methods. Scrambled siRNA sequence used to account for nonspecific, off-target effects. To account for differences in protein loading between lanes, final PDGFR-β knockdown was reported as percentage of actin-normalized PDGFR-β band intensity in the siRNA treated lane relative to actin-normalized PDGFR-β band intensity in the control (vehicle-treated) lane using computer image analysis software with a gel analysis package (ImageJ v1.47, NIH, Bethesda, MD)