Table 4 Validation parameters: accuracy, intra-day and inter-day precision, matrix effects. N/A, not determined
Analyte | QC sample | Accuracy, bias (%) | Intra-day precision, SD (%) | Inter-day precision, SD (%) | ME, mean ± SD (%) |
|---|---|---|---|---|---|
Morphine | Low | 0.6 | 6.2 | 8.8 | 56 ± 6.9 |
Medium | 3.2 | 3.5 | 3.8 | N/A | |
High | −0.1 | 3.4 | 3.4 | 53 ± 9.8 | |
Tramadol | Low | 2.4 | 4.3 | 5.2 | 107 ± 21 |
Med | 0.4 | 4.5 | 4.5 | N/A | |
High | 4.9 | 1.8 | 2.3 | 183 ± 90 | |
Detomidine | Low | 3.9 | 7.6 | 7.7 | 88 ± 24 |
Medium | 5.9 | 7.7 | 9.1 | N/A | |
High | 2.9 | 4.5 | 5.9 | 79 ± 12 | |
Butorphanol | Low | −0.4 | 10.2 | 15.2 | 87 ± 12 |
Medium | 1.9 | 4.5 | 4.5 | N/A | |
High | −1.8 | 4.0 | 6.6 | 84 ± 10 | |
Buprenorphine | Low | −9.3 | 4.5 | 8.0 | 87 ± 23 |
Medium | −1.2 | 4.5 | 4.5 | N/A | |
High | −0.6 | 2.0 | 2.0 | 88 ± 7.7 | |
Acepromazine | Low | 87 | 28 | 53 | 97 ± 31 |
Medium | 8.3 | 8.2 | 11 | N/A | |
High | −0.7 | 11 | 11 | 117 ± 14 | |
Firocoxib | Low | 4.7 | 3.0 | 6.7 | 82 ± 11 |
Medium | 2.6 | 1.7 | 2.0 | N/A | |
High | 2.8 | 4.0 | 4.0 | 83 ± 8.7 | |
Meloxicam | Low | 4.1 | 2.9 | 5.7 | 78 ± 12 |
Medium | 4.2 | 3.1 | 5.2 | N/A | |
High | −4.1 | 3.1 | 3.5 | 70 ± 12 | |
Chlorpromazine | Low | −3.5 | 3.2 | 6.3 | 77 ± 42 |
Medium | 6.2 | 2.9 | 2.9 | N/A | |
High | 0.9 | 2.9 | 3.2 | 90 ± 41 | |
Ketoprofen | Low | 6.1 | 5.6 | 7.0 | 78 ± 13 |
Medium | −1.9 | 1.8 | 2.1 | N/A | |
High | 5.3 | 5.5 | 5.5 | 86 ± 12 | |
Flunixin | Low | 5.8 | 5.8 | 7.3 | 89 ± 16 |
Medium | 0.4 | 2.2 | 2.8 | N/A | |
High | 3.5 | 3.2 | 3.2 | 75 ± 7.0 | |
Fluphenazine | Low | 7.3 | 5.6 | 14.8 | 92 ± 125 |
Medium | 12.2 | 20 | 21 | N/A | |
High | 18.4 | 18.7 | 19.3 | 81 ± 51 | |
Phenylbutazone | Low | 6.1 | 9.8 | 10.7 | 75 ± 14 |
Medium | 1.2 | 4.4 | 6.1 | N/A | |
High | 2.3 | 4.6 | 6.2 | 60 ± 6.7 |