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Table 2 LC-MS/MS parameters

From: Long-term monitoring of opioid, sedative and anti-inflammatory drugs in horse hair using a selective and sensitive LC-MS/MS procedure

Analyte/internal standard

Precursor ion (m/z)

Product ion (m/z)

Relative ion intensities (mean) ± rSD (%)a

RT (min)

DP (V)

EP (V)

CE (V)

CxP (V)

Morphine

286.1

152.1

0.7 ± 16

0.6

156

10

81

14

128.1

0.6

156

10

79

16

165.1

0.6

156

10

57

16

Tramadol

264.05

58.0

0.32 ± 6.2

3.4

46

10

17

10

58.5

3.4

46

10

33

8

42.0

3.4

46

10

115

20

Detomidine

187.09

81.1

0.23 ± 7.7

3.9

101

10

25

8

54.1

3.9

101

10

55

8

Butorphanol

328.17

310.2

0.15 ± 3.6

4.2

101

10

33

22

157.0

4.2

101

10

59

12

131.1

4.2

101

10

65

12

Buprenorphine

468.24

55.1

0.05 ± 12

5.1

1

10

101

6

41.1

5.1

1

10

129

4

115.1

5.1

1

10

129

6

Acepromazine

327.04

86.0

0.99 ± 5.6

5.5

81

10

25

10

58.0

5.5

81

10

65

8

222.1

5.5

81

10

53

16

Firocoxib

337.02

283.1

0.81 ± 7.1

5.6

101

10

13

16

130.1

5.6

101

10

41

12

237.0

5.6

101

10

23

20

Meloxicam

351.96

115.0

0.37 ± 7.0

6.3

126

10

25

10

141.0

6.3

126

10

27

12

73.0

6.3

126

10

75

12

Chlorpromazine

319.26

58.1

0.89 ± 4.2

6.7

86

10

67

12

86.1

6.7

86

10

25

8

214.0

6.7

86

10

57

20

Ketoprofen

255.08

77.0

0.93 ± 6.7

6.7

131

10

61

12

104.9

6.7

131

10

33

12

51.1

6.7

131

10

99

8

Flunixin

297.03

278.9

0.38 ± 3.1

7.3

111

10

33

24

264.0

7.3

111

10

47

22

236.1

7.3

111

10

57

16

Fluphenazine

438.22

171.0

0.71 ± 2.8

9.3

56

10

37

20

143.1

9.3

56

10

37

18

70.0

9.3

56

10

67

10

Phenylbutazone

309.15

77.0

0.51 ± 9.6

9.5

216

10

75

12

160.1

9.5

216

10

29

14

120.0

9.5

216

10

27

10

Morphine-d3

289.02

201.0

 

0.6

156

10

35

16

152.1

0.6

156

10

75

18

13C-Tramadol-d3

268.07

58.0

3.4

46

10

49

14

42.1

3.4

46

10

113

20

Buprenorphine-d4

472.26

59.0

5.1

1

10

107

6

400.1

5.1

1

10

53

2

Chlorpromazine-d3

322.04

89.1

6.7

51

10

25

10

61.1

6.7

51

10

59

8

Ketoprofen-d3

258.09

212.1

6.7

121

10

19

14

105.0

6.7

121

10

31

10

13C12-Phenylbutazone

321.11

166.2

9.5

76

10

29

14

55.1

9.5

76

10

129

8

  1. MRM (multiple reaction monitoring mode) transitions with precursor and product ions, relative ion intensities of MRM transitions (qualifier-to-quantifier); RT retention time, DP declustering potential, EP entrance potential, CE collision energy, CxP collision cell exit potential. MRM transitions used as quantifiers are given in bold
  2. adetermined from calibrators (n = 10, per analyte)
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BMC Veterinary Research

ISSN: 1746-6148

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