Fig. 2

The effect of glucose concentration on cell growth and lactose synthesis. a, b The effect of glucose concentration on cell viability (a) and proliferation (b) in dairy cow mammary epithelial cells. c The effect of glucose concentration on lactose synthesis in dairy cow mammary epithelial cells. Dairy cow mammary epithelial cells were cultured in DMEM with glucose at concentrations ranging from 0 to 20 mM. The effects of glucose concentration on cell viability, proliferation, and lactose content were examined after 12, 24, 36, and 48 h of treatment. Data are shown as the mean ± SEM from 3 independent replicates. *P < 0.05; **P < 0.01 versus corresponding value for cells cultured in DMEM with 0 mM glucose