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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Effects of glucose on lactose synthesis in mammary epithelial cells from dairy cow

Fig. 1

Glucose induces dairy cow mammary epithelial cell growth and lactose synthesis. a Mammary epithelial cells isolated from mid-lactating mammary tissues of dairy cows were acquired using a phase-contrast light microscopy with a Leica L 40 × 0.5 PH2 objective. b Immunofluorescence staining for cytokeratin 18 in dairy cow mammary epithelial cells was acquired using a confocal microscopy with a Leica HCX PL Apo CS 40 × 1.25 oil objective. Cytokeratin 18 was stained with FITC (green), and nuclei were stained with propidium iodide (red). For A and B, Scale bar, 75 μm. c, d The effect of glucose on cell viability (c) and proliferation (d) in dairy cow mammary epithelial cells. e Lactose secretion from dairy cow mammary epithelial cells cultured with or without glucose. Lactose content in medium was measured with the Lactose/d-Glucose (Rapid) Assay kit (K-LACGAR, Megazyme, Ireland, UK). For c, d, and e, mammary epithelial cells were cultured in DMEM with high glucose (+Glucose, 25 mM) or without glucose (-Glucose) for 24 h. Data are shown as the mean ± SEM from 3 independent replicates. *P < 0.05, **P < 0.01

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