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Table 2 LCDV detection and estimated viral load determined by real-time PCR in caudal fin from diseased and asymptomatic gilthead seabream juveniles

From: Application of a new real-time polymerase chain reaction assay for surveillance studies of lymphocystis disease virus in farmed gilthead seabream

   

Viral loadd

Fish populationa

LCD signsb

LCDV detectionc

Load range

Mean ± SD

A

-

73.3 (15)

1–15

(4 ± 3.99) x 100

A

+

100 (6)

1.2 x 105–6.6 x 105

(3.53 ± 2.77) x 105

B

-

30.0 (10)

1–330

(1.1 ± 1.89) x 102

B

+

100 (5)

2.9 x 104–6.9 x 104

(5 ± 1.75) x 104

C

-

87.5 (16)

1.7–54

(1.6 ± 1.56) x 101

D

-

100 (8)

12–140

(5.3 ± 5.88) x 101

  1. a A, B, C and D are four gilthead seabream farms located in the Mediterranean area. Only farm C had no previous reports of lymphocystis disease (LCD)
  2. b Typical lymphocystis disease signs: +, presence; -, absence
  3. c Percentage of LCDV-positive fish (total number of fish analysed)
  4. d Copies of viral DNA per mg of tissue
  5. SD standard deviation