Table 1 Intra- and inter-assay variability of the real-time PCR

1 x 10⁵

19.92 ± 0.16

0.78

18.92 ± 0.54

2.83

1 x 10⁴

23.34 ± 0.17

0.73

22.27 ± 0.60

2.69

1 x 10³

26.87 ± 0.18

0.66

26.14 ± 0.46

1.75

1 x 10²

30.22 ± 0.27

0.89

29.49 ± 0.71

2.39

1.6 x 10¹

32.15 ± 0.47

1.45

32.22 ± 0.87

2.72

4 x 10⁰

34.15 ± 0.80

2.34

33.37 ± 0.89

2.68

2 x 10⁰

35.23 ± 0.99

2.80

34.09 ± 1.14

3.35

Overall CV^c (%)

1.38 ± 0.87

2.63 ± 0.48