Fig. 6

The effect of siRNAs on IRES-mediated translation in CPK cells. After 48 h of siRNA treatment, cells were further transfected with plasmid construct. 24 h following transfection, Renilla luciferase and firefly luciferase units were measured using the Dual-Luciferase Reporter Assay System and IRES-mediated translation activity in CPK cells. Experiments were performed in triplicates and error bars indicate S.D