Fig. 3From: IRES-mediated translation of foot-and-mouth disease virus (FMDV) in cultured cells derived from FMDV-susceptible and -insusceptible animalsThe effect of siRNAs on the cellular factors. The siRNAs targeting ITAF45, PTB, and 4E-BP1 were reverse transfected into MDCK, MDBK, and CPK cells using Lipofectamine RNAiMAX, and incubated for 48 h. After 48-h treatment, western blot analysis was performed on cell lysates to confirm the effect of siRNAs. Protein bands were quantified using an LAS1000UVmini. The protein intensity was divided by that of actin, and the normalized ratio (100 %) was indicatedBack to article page