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Fig. 1 | BMC Veterinary Research

Fig. 1

From: IRES-mediated translation of foot-and-mouth disease virus (FMDV) in cultured cells derived from FMDV-susceptible and -insusceptible animals

Fig. 1

pRF/FMDV-IRES and pCAGGS/FMDV-IRES plasmid construction. Structure of the bicistronic luciferase reporter construct containing the FMDV-IRES element located between Renilla luciferase and firefly luciferase (pRF-FMDV-IRS). Reporter gene was excised from this plasmid construct using the restriction enzymes EcoRV and HpaI, and was o the pCAGGS/MCS(F) vector treated with SmaI and rAPid Alkaline Phosphatase using Mighty Mix

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