Fig. 5From: In situ detection of GM1 and GM2 gangliosides using immunohistochemical and immunofluorescent techniques for auxiliary diagnosis of canine and feline gangliosidosesImmunofluorescent findings for the detection of GM2 ganglioside in animals affected and unaffected with gangliosidoses. The immunofluorescent technique for the detection of GM2 ganglioside was performed on paraffin-embedded sections of the cerebral cortex from the following animals: a dog (a) and a cat (b) affected with GM1 gangliosidosis; a dog (c) and a cat (d) affected with GM2 gangliosidosis; an unaffected control dog (e) and cat (f). For the detection of GM2 ganglioside, mouse anti-GM2 monoclonal IgM antibody was used as a primary antibody, and Alexa Fluor 488-conjugated goat anti-mouse IgM antibody was used as a secondary antibody. Subsequently, these sections were incubated with 4′,6-diamidino-2-phenylindole dihydrochloride for nuclear staining. Bar = 30 μmBack to article page