Skip to main content
Fig. 5 | BMC Veterinary Research

Fig. 5

From: In situ detection of GM1 and GM2 gangliosides using immunohistochemical and immunofluorescent techniques for auxiliary diagnosis of canine and feline gangliosidoses

Fig. 5

Immunofluorescent findings for the detection of GM2 ganglioside in animals affected and unaffected with gangliosidoses. The immunofluorescent technique for the detection of GM2 ganglioside was performed on paraffin-embedded sections of the cerebral cortex from the following animals: a dog (a) and a cat (b) affected with GM1 gangliosidosis; a dog (c) and a cat (d) affected with GM2 gangliosidosis; an unaffected control dog (e) and cat (f). For the detection of GM2 ganglioside, mouse anti-GM2 monoclonal IgM antibody was used as a primary antibody, and Alexa Fluor 488-conjugated goat anti-mouse IgM antibody was used as a secondary antibody. Subsequently, these sections were incubated with 4′,6-diamidino-2-phenylindole dihydrochloride for nuclear staining. Bar = 30 μm

Back to article page