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Fig. 3 | BMC Veterinary Research

Fig. 3

From: In situ detection of GM1 and GM2 gangliosides using immunohistochemical and immunofluorescent techniques for auxiliary diagnosis of canine and feline gangliosidoses

Fig. 3

Immunohistochemical findings for the detection of GM2 ganglioside in animals affected and unaffected with gangliosidoses. The immunohistochemical technique for the detection of GM2 ganglioside was performed on paraffin-embedded sections of the cerebral cortex from the following animals: a dog (a) and a cat (b) affected with GM1 gangliosidosis; a dog (c) and a cat (d) affected with GM2 gangliosidosis; an unaffected control dog (e) and cat (f). For the detection of GM2 ganglioside, mouse anti-GM2 ganglioside monocloncal IgM antibody was used as a primary antibody, and biotinylated goat anti-mouse IgM antibody was used as a secondary antibody. Subsequently, these sections were incubated with peroxidase-labeled streptavidin. The immunoreactivity was detected by 3,3′-diaminobenzidine as a peroxidase substrate. The sections were counterstained with hematoxylin. Bar = 50 μm

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