Fig. 3From: In situ detection of GM1 and GM2 gangliosides using immunohistochemical and immunofluorescent techniques for auxiliary diagnosis of canine and feline gangliosidosesImmunohistochemical findings for the detection of GM2 ganglioside in animals affected and unaffected with gangliosidoses. The immunohistochemical technique for the detection of GM2 ganglioside was performed on paraffin-embedded sections of the cerebral cortex from the following animals: a dog (a) and a cat (b) affected with GM1 gangliosidosis; a dog (c) and a cat (d) affected with GM2 gangliosidosis; an unaffected control dog (e) and cat (f). For the detection of GM2 ganglioside, mouse anti-GM2 ganglioside monocloncal IgM antibody was used as a primary antibody, and biotinylated goat anti-mouse IgM antibody was used as a secondary antibody. Subsequently, these sections were incubated with peroxidase-labeled streptavidin. The immunoreactivity was detected by 3,3′-diaminobenzidine as a peroxidase substrate. The sections were counterstained with hematoxylin. Bar = 50 μmBack to article page