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Table 3 Average (± standard deviation SD) values of macroscopic and histopathological lung lesion scores, IF scores, and qPCR

From: Efficacy of Mycoplasma hyopneumoniae vaccination before and at weaning against experimental challenge infection in pigs

Parametera V1 (n = 20) V2 (n = 19) PCG (n = 19) P value
Macroscopic lung lesions 0.54 ± 0.67A 0.88 ± 1.45A 1.04 ± 2.45A 0.777
Lymphohistiocytic infiltration 2.95 ± 0.50A 3.16 ± 0.58B 3.61 ± 0.59C 0.002
Percentage of air in lung tissue 44.28 ± 14.68A 37.47 ± 17.76B 31.46 ± 14.51C 0.000
IF 1.13 ± 0.54A 1.19 ± 0.79A 1.25 ± 0.70A 0.896
qPCR on BAL fluid 102.94 ± 101.24A 102.76 ± 101.48A 103.23 ± 101.33A 0.616
  1. Treatment groups: V1 vaccinated before weaning, at 18 days of age, challenge infected, V2 vaccinated at weaning, at 21 days of age, challenge infected, and PCG positive control group i.e. non-vaccinated, challenge infected
  2. Values with different superscript (A-C) in capital letter within a row are significantly different (P ≤ 0.05)
  3. a Macroscopic lung lesion scoring based on Hannan et al. [30]
  4. Lymphohistiocytic infiltration score based on Morris et al. [31]
  5. Percentage of air in lung tissue measured by means of automatic image analysis system (Optimas® 6.5, Media Cybernetics, Silver Spring, USA)
  6. IF scoring based on Kobisch et al. [32]
  7. Values from qPCR on BAL fluid expressed as copies of M. hyopneumoniae/mL