BMC Veterinary Research

Table 3 Concentrations (mmol/L) of volatile fatty acids at 6 and 24 h of the vitro study^a

From: In vitro influence of dietary protein and fructooligosaccharides on metabolism of canine fecal microbiota

							ANOVA P-value
	LP HD	HP HD	HP LD	LP HD + FOS	HP HD + FOS	HP LD + FOS	Protein level	Protein digestibility	FOS	Pooled SEM
6 h
Acetic acid	15.2	17.6	9.7	16.8	11.2	8.8	0.304	0.005	0.220	1.44
Propionic acid	1.58	1.56	1.09	1.04	0.59	0.52	0.236	0.214	<0.001	0.18
iso-Butyric acid	0.16	0.15	0.12	0.10	0.02	0.03	0.246	0.731	0.011	0.04
n-Butyric acid	2.81	3.10	1.04	7.47	2.33	2.12	0.040	0.304	0.052	1.04
iso-Valeric acid	0.03	0.01	0.04	0.01	0.01	0.01	0.207	0.304	0.135	0.01
Total VFA	19.8	22.4	12.0	25.5	14.2	11.4	0.090	0.019	0.809	2.30
C2:C3	10.3	11.8	9.1	16.4	23.8	26.9	0.457	0.879	0.030	5.53
C2 + n-C4:C3	12.3	13.7	10.0	23.6	28.1	30.3	0.616	0.997	0.005	5.43
24 h
Acetic acid	27.4	24.2	18.8	28.1	33.2	21.8	0.862	0.003	0.037	2.23
Propionic acid	7.65	6.37	1.93	12.12	10.88	7.86	0.105	<0.001	<0.001	0.66
iso-Butyric acid	0.09	0.52	0.16	0.27	0.09	0.27	0.201	0.500	0.483	0.11
n-Butyric acid	2.12	3.79	1.11	9.72	7.97	10.67	0.880	0.848	<0.001	0.90
iso-Valeric acid	0.44	0.68	0.23	0.44	0.11	0.22	0.950	0.303	0.106	0.14
Total VFA	37.7	35.5	22.3	50.6	52.3	40.8	0.864	0.001	<0.001	2.66
C2:C3	3.65	3.81	9.61	2.35	3.07	2.77	0.585	0.006	<0.001	0.70
C2 + n-C4:C3	3.91	4.40	10.22	3.14	3.86	4.13	0.409	0.001	0.001	0.65

LP HD low protein high digestibility, HP HD high protein high digestibility, HP LD high protein low digestibility, FOS fructooligosaccharides, VFA volatile fatty acids, C2/C3 acetic acid/propionic acid ratio, C2 + n-C4/C3 acetic acid + n-butyric acid/propionic acid ratio
^aValues are the means of five bottles per treatment

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