Skip to main content
Fig. 4 | BMC Veterinary Research

Fig. 4

From: Construction of a camelid VHH yeast two-hybrid library and the selection of VHH against haemagglutinin-neuraminidase protein of the Newcastle disease virus

Fig. 4

Detection of interaction between selected VHH and NDV virion by ELISA. Microplates were coated with NDV La Sota virion (TCID50 10-8.17/0.1 mL, 1:20 dilution). The 7 selected VHH (200 μg/mL) were diluted at different concentrations, and anti-NDV positive serum (Positive control), negative serum (Negative control 1) and 6 × His SUMO tag (Negative control 2) were used as positive and negative control. The value of absorbance at 450 nm indicate the reactivity between VHH and NDV. And the data represents the average of 3 wells

Back to article page