Table 3 Primers used for amplification and sequencing of feline bocavirus (design/numbering based on GenBank accession number KM017745) and for confirmation of the substitution in NS1 coding sequence of FPV (design/numbering based on GenBank accession number KP769859)
From: Feline panleukopenia virus in cerebral neurons of young and adult cats
Forward Primer | Sequence | Position | Reverse primer | Sequence | Position |
|---|---|---|---|---|---|
BocaF1 | ACAATGCCTGGACCTGAATC | 481 → 500 | BocaR1 | TTTTACTCATTCTGGCATTCACA | 1,276 → 1,254 |
BocaF2 | CTAGTCAGGGACGAACCAA | 1,141 → 1,159 | BocaR2 | CCATTGAGTATGAAAGCCAACT | 2,103 → 2,082 |
BocaF3 | CACGTCTAGAGAGCACCTTTGG | 1,994 → 2,015 | BocaR3 | ATCAACCTCCATGGCAACC | 3,118 → 3,100 |
BocaF4 | DGGCATTTTAGCATGGGCGAA | 2,966 → 2,986 | BocaR4 | GAAAATACCCGTATTGCGGAAGT | 4,113 → 4,091 |
BocaF5 | TACAGCGGGTGTACACATTT | 3,9864,005 | BocaR5 | GTAGCAGTGTGGAGGGTGT | 5,368 → 5,350 |
ParvF3 | CAACCAATAAGAGACAGAATGTTGA | 1,785 → 1,809 | ParvR3 | CCCCCACTTTACTAACACACC | 2,342 → 2,322 |